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Image Search Results
Journal: The Journal of Neuroscience
Article Title: Ribozyme-Mediated Inhibition of Caspase-3 Protects Cerebellar Granule Cells from Apoptosis Induced by Serum–Potassium Deprivation
doi: 10.1523/JNEUROSCI.20-01-00179.2000
Figure Lengend Snippet: Apoptosis after 24 hr of serum–K+ deprivation in CGCs expressing positive and negative control vectors. β-Galactosidase, a negative control, and human bcl-2 or p35, positive controls, were expressed transiently in CGCs. After 24 hr of deprivation of serum and K+, the percentage of apoptotic cells was determined as described in Materials and Methods. Apoptosis measured 44 ± 2% in negative control cells (average cells counted, x = 241;n = 10), 5 ± 2% with human bcl-2 (x = 88; n = 3), and 29 ± 1% with p35 (x = 186; n = 7). ***p < 0.001 compared with negative control by ANOVA and one-tailed Dunnett's post hoc test.
Article Snippet: Coding sequences for β-galactosidase, human bcl-2, p35, and RZ101 were cloned into pCR3.1 (Invitrogen, San Diego, CA). β-Galactosidase was excised from pIND-lacZ (Invitrogen) using Hin dIII and Xho I; human bcl-2 was excised from pB4 (American Type Culture Collection, Manassas, VA) using Eco RI; and p35 was excised from
Techniques: Expressing, Negative Control, One-tailed Test